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  • 商品編號 ID:Sengenics

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KREX™ – World’s only proteomics technology that produces full-length, correctly folded, functionally verified proteins

Sengenics is a functional proteomics company that leverages its patented KREXTM technology for production of full-length, correctly folded and functional proteins. KREXTMutilises the Biotin Carboxyl Carrier Protein (BCCP) folding marker which is cloned in-frame with the gene encoding the protein of interest. BCCP acts not only as a protein folding marker but also as a protein solubility enhancer.

BCCP is fused to either the N- or C-terminal of a protein of interest. Full-length proteins are expressed as fusions to the BCCP folding marker which becomes biotinylated in vivo, BUT only when the protein is correctly folded. Conversely, misfolded proteins drive the co-translational misfolding of BCCP to such an extent that it becomes catalytically inactive, and is unable to become biotinylated. Hence, misfolded proteins no longer have a way of attaching to a streptavidin-coated solid support. This is the fundamental principle of the KREXTM technology. Only correctly folded proteins become attached to a solid support.

As biotinylated proteins bound to a streptavidin-coated surface show negligible dissociation, this interaction therefore provides a vastly superior means for tethering proteins to a planar surface and is ideal for applications such as protein arrays, SPR and bead-based assays. The use a compact, folded, biotinylated, 80 residue domain BCCP affords two significant advantages over the AviTag and intein-based tag. First, the BCCP domain is cross-recognised by eukaryotic biotin ligases enabling it to be biotinylated efficiently in yeast, insect, and mammalian cells without the need to co-express the E. coli biotin ligase. Second, the N- and C-termini of BCCP are physically separated from the site of biotinylation by 50Å (Fig. 1), so the BCCP domain can be thought of as a stalk which presents the recombinant proteins away from the solid support’s surface, thus minimising any deleterious effects due to immobilisation.

更多詳細資料請參閱 : http://www.sengenics.com/krex/




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